SleepStats 4 Pro version for rats or mice is our latest upgrade, featuring a compact interface for characterizing and viewing sleep-wake behavior patterns from data created by PiezoSleep. It also includes options to characterize 3-state vigilance behaviors (Wake, NREM, and REM), activity levels, and an expanded set of CSV export functions. In addition to viewing and exporting data from our environmental sensor, it also has options to use the light levels to automatically segment out light and dark cycles when computing phase-based statistics. This is useful when applying non-periodic light-dark cycles.
This is a premium version and requires an activation code to run the software. Contact Signal Solutions if you are interested in upgrading from SleepStats 2. x, or need to install a copy on an additional PC.
For customers who already have SleepStats 4 Pro, a link to download the install script for the latest update is found below. If a validated version is already installed on the computer, the install script below will automatically update and validate the program. If it is a new installation or new computer, an activation code will need to be entered when the program is first run after the installation.
Download, unzip, and run the executable.
WAKE, REM, NREM Classification File Creator:
For vigilance 3-state analyses, a special program must be applied to the binfb files to generate a file that classifies sleep states into REM and NREM. As of now, this is only for mice (a rat version has NOT been developed/validated) and requires a good signal-to-noise ratio in the recorded data such that the signals from respiration can be consistently observed. The REM detection algorithm relies on changes breathing patterns, which may be obscured by noise levels (external platform/cage vibrations or cross-talk) or the respiration signal may not be strong enough (excess bedding or a very or weak animal). Therefore, it is recommended only for use with our film sensor (custom cage) systems and on recordings greater than 24 hours (for robust calibration). The install script for generating the 3-state files (REM_NREM_WAKE) can be downloaded from the button below.
Download, unzip, and run the executable.
When the program is running, an instruction window appears. When OK is checked, a prompt appears to select the recording files to process will appear. Multiple files can be selected. The program requires about 90 seconds per mouse per day to run on a typical PC, so for large files or many files, this may run for several hours. When finished, the 3-state file (wss extension) will be saved in the same folder as the selected binfb file. This must be in the same directory as the other recorded files for SleepStats 4 to load it and present an option for 3-state analysis.
Want to learn more? Click to watch SleepStats 4 Pro video. The manual for the full version of SleepStats 4 can be found here
